王瑞副研究员，全职科研人员

副研究员

wangr@fudan.edu.cn

  工作经历

2024.12-今 复旦大学人类表型组研究院 副研究员，硕士生导师

上海国际人类表型组研究院，青年副研究员（兼聘）

复旦大学附属浦东医院，副研究员（兼聘）

2021.9-2024.12 复旦大学人类表型组研究院，青年副研究员，硕士生导师

2019.9-2021.9   复旦大学，博士后

2013.9-2019.6  浙江大学，博士

2017.11-2018.11 哈佛大学，联合培养博士研究生

  研究方向

王 瑞，上海市“启明星”人才，复旦大学青年副研究员，硕士生导师。博士毕业于浙江大学，在哈佛大学交流学习一年。主要研究领域为：新型分子诊断方法开发和智能生物传感器构建。已主持国家级和省部级项目8项，包括：国家自然科学基金面上项目，国家自然科学基金青年项目，上海市重大专项（二期）子项目，上海市“启明星”人才项目，上海市实验动物研究专项，粤港澳精准医学专项、博士后面上基金，新冠疫情防控专项等，总科研经费350万以上。已经在本领域Top期刊Cell Discov., Biosens. Bioelectron., Theranostics, Trend Anal. Chem., Anal. Chem.等期刊上发表SCI论文46篇，其中以第一作者或通讯作者（含共同）发表SCI论文27篇，他引2000余次，2篇被评为“ESI高被引文章”，已申请发明专利16项。研究成果主要包括：（1）自主开发和改造新型CRISPR/Cas酶工具，用于基因编辑和分子诊断；（2）开发了多种CRISPR结合快速核酸扩增的高敏特异分子诊断方法，实现了病原体感染快检和癌症早筛；（3）开发了微流控平台和集成化便携式装备，实现了精准定量和POCT即时检测。主要研究方向：生物传感、纳米材料、新型核酸扩增方法开发、CRISPR分子诊断、微流控、数字PCR等技术领域。

  招生专业

生物学（研究方向：分子生物学、遗传学、分子流行病、生物医药等）

  获奖情况

国家自然科学基金面上项目（2023）

国家自然科学基金青年基金 (2020)

上海市重大专项（二期）子项目（2025）

上海市“启明星”人才项目（2023）

上海市“科技创新行动计划”实验动物研究专项(2023)

粤港澳大湾区精准医学专项(2024)

中国博士后科学基金第67批面上资助 (2020)

中国博士后科学基金新冠肺炎疫情防控专项资助 (2020)

国家建设高水平大学公派研究生联合培养博士生项目资助（2017）

  代表论文

  1. Linlin Bai, Yanan Pang, Ting Wang, Shengzhou Wang, Kaiming Guo, Tian Xuan, Ziqin Zhang, Dianwei Liu, Feng Qian, Yan Zheng, Gang Jin*, Rui Wang*. SPEAR: CRISPR-mediated ultrasensitive, specific and rapid one-pot detection strategy for cancer-related SNPs. Theranostics, 2025,15(8):3275-3288. (中科院一区TOP, IF=12.4)

  2. Huilin Mao, Yuwen Tian, Ziwen Wang, Jingtong Liu, Jingjing Wei, Yao Wang, Chen Tao, Miaomiao Li, Shengzhou Wang, Junnan Tang, Rui Wang*, Song Gao*, Feng Lan*, Yongming Wang*. Discovery and engineering of Tsp2Cas9 for genome editing. Cell Discovery, 2024, 10,55. (中科院一区TOP, IF=13).

  3. Rui Wang, Chunyan Qian, Yanan Pang, Miaomiao Li, Yu Yang, Haijing Ma, Mengying Zhao, Feng Qian, Hang Yu, Zhenping Liu, Ting Ni*, Yan Zheng*, Yongming Wang*. opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-CoV-2 detection. Biosensors and Bioelectronics, 2021, 172:112766. (中科院一区TOP，IF=10.7) (高被引文章)

  4. Yongkang Lai, Kaiming Guo, Chunping Zhu, Yanwei Lv, Hang Wu, Liang Zhang, Mengni Jiang,  Jiulong Zhao*, Rui Wang*. Cas14VIDet: A visual instant method free from PAM restriction for antibiotic resistance bacteria detection, Biosensors and Bioelectronics, 2024,116884. (中科院一区TOP，IF=10.7).

  5. Ting Wang, Ziwei Wang, Linlin Bai, Xingcai Zhang, Hongyu Zhang, Jia Feng*, ChengQian*, Yongming Wang*, Rui Wang*. Next-generation CRISPR-based diagnostic tools for human diseases. Trends in Analytical Chemistry, 2023, 117328 (中科院一区TOP，IF=11.8).

  6. Kaiming Guo, Yongkang Lai, Linlin Bai, Ting Wang, Shengzhou Wang, Ziqin Zhang, Dianwei Liu, Mingrui Zhu, Jiulong Zhao, Yanan Pang, Feng Qian, Yongming Wang, Rui Wang*, SIMPLE: A one-pot RPA-Cas12b method for SNVs identification with an expanded scope and clinical applications, Phenomics, 2025, doi: https://doi.org/10.1007/s43657-025-00240-x.（中科院一区, IF=6.2）

  7. Ting Wang, Linlin Bai, Guoling Wang, Jingli Han, Lixin Wu, Xuanzhong Chen, Hongyu Zhang*, Jia Feng*, Yongming Wang*, Rui Wang*, Xiaohui Zhang*. SATCAS: a CRISPR/Cas13a-based simultaneous amplification and testing platform for one-pot RNA detection and SNPs distinguish in clinical diagnosis. Biosensors and Bioelectronics, 2024,263,116636. (中科院一区TOP, IF=10.7) .

  8. Ziqin Zhang, Cui Wu, Linlin Bai, Kaiming Guo, Ting Wang, Dianwei Liu, Mingrui Zhu, Feng Qian, Fang Zhang*, Rui Wang*, MVPCR: Multiplex visual detection strategy based on ultrafast PCR for point-of-care pathogens detection within 10 min, Phenomics, 2025, https://doi.org/10.1007/s43657-024-00216-3.(中科院一区, IF=6.2）

  9. Jungang Du, Jinsong He, Rui Wang* , Jian Wu*, Xiaoping Yu*. Ultrasensitive reporter DNA sensors built on nucleic acid amplification techniques: Application in the detection of trace amount of protein. Biosensors and Bioelectronics, 2023, 115761. (中科院一区TOP，IF=10.7).

  10. Shuaiwei Chen, Cui Wu, Chunyan Qian, Yanan Pang, Kaiming Guo, Ting Wang, Linlin Bai, Feng Qian, Zunzhong Ye, Zhenping Liu, Zhaohui Qiao*, Yongming Wang*, Rui Wang*. Ultraspecific one-pot CRISPR-based Green-Yellow-Red multiplex detection strategy integrated with portable cartridge for point-of-care diagnosis. Analytical Chemistry, 2024, 96, 7, 3145-3152. (中科院一区TOP, IF=6.7).

  11. Rui Wang, Yongfang Li*, Yanan Pang*, Fang Zhang, Fuyou Li, Shihua Luo* and Chunyan Qian*. VIR-CRISPR: Visual in-one-tube ultrafast RT-PCR and CRISPR method for instant SARS-CoV-2 detection. Analytica Chimica Acta, 2022, 1212:339937 (中科院一区TOP，IF=5.7).

  12. Yanju Chen, Yuanyuan Zhu, Xiaofu Wang, Cheng Peng, Rui Wang* , Tingzhang Wang, Yang Che, Jian Wu*, Junfeng Xu*, Gravity-driven and rotation-controlled microfluidicchip for point-of-care nucleic acid detection in the fully closed environment, Talanta, 2024, 267,125258. (中科院一区TOP, IF=5.6) .

  13. Yuanyuan Zhu, Sihan Ma, Jungang Du, Yanju Chen, Qingli Zhou*, Rui Wang*, Jian Wu*. μLAMP-LFA: A nucleic acid analysis platform for the field diagnosis of pathogens in aquatic animals, Analytica Chimica Acta, 2025, 1360 (2025) 344144. (中科院一区TOP, IF=5.7).

  14. Zhaohui Qiao, Liangliang Xue, Mengni Sun, Min Zhang, Min Chen, Xia Xu, Wenge Yang, Rui Wang*. Highly sensitive detection of Salmonella based on dual-functional HCR-mediated multivalent aptamer and amplification-free CRISPR/Cas12a system, Analytica Chimica Acta, 2023, 1284:341998. (中科院一区TOP，IF=5.7) .

  15. Rui Wang, Ying Liu, Shuaiwei Chen, Linlin Bai, Kaiming Guo, Yanan Pang, Feng Qian, Yongfang Li, Li Ding*, Yongming Wang*. utPCR: A strategy for the highly specific and absolutely quantitative detection of single molecules within only minutes. Biosensors, 2023, 13:10 (中科院二区, IF=5.4) .

  16. Bei Wang#, Rui Wang#, Daqi Wang, Jian Wu, Jixi Li, Jin Wang, Huihui Liu, and Yongming Wang*. Cas12aVDet: a CRISPR/Cas12a-based platform for rapid and visual nucleic acid detection. Analytical Chemistry, 2019, 91(19):12156-12161. (中科院一区TOP,  IF=7.4) (高被引文章)

  17. Rui Wang, Rui Chen, Cheng Qian, Yanan Pang, Jian Wu and Fuyou Li*. Ultrafast Visual Nucleic Acid Detection with CRISPR/Cas12a and Rapid PCR in Single Capillary. Sensors and Actuators B-Chemical, 2021, 326:128618. (中科院一区TOP,  IF=9.22)

  18. Rui Wang, Fang Zhang, Liu Wang, Wenjuan Qian, Cheng Qian, Jian Wu* and Yibin Ying. Instant, Visual, and Instrument-Free Method for On-Site Screening of GTS 40-3-2 Soybean Based on Body-Heat Triggered Recombinase Polymerase Amplification. Analytical Chemistry, 2017, 89(8), 4413-4418.(中科院一区TOP,  IF=7.4)

  19. Yongfang Li, Zijun Wang, Xuan Chen, Zhijian Yi, Rui Wang*. In situ deposition of bismuth on pre-anodized screen-printed electrode for sensitive determination of Cd2+ in water and rice with a portable device, Scientific Reports, 2024, 14:18433. (中科院二区，IF=3.8) .

  20. Yongfang Li, Xuan Chen, Zhiyong Yuan, Zhijian Yi, Zijun Wang, Rui Wang*, Nanoporous gold modified screen-printed electrode for simultaneous determination of Pb2+ and Cu2+ in water, Sensors, 2024. (中科院二区，IF=3.4).

  21. Rui Wang, Fang Zhang, Cheng Qian, Cui Wu, Zunzhong Ye, Liu Wang, Wenjuan Qian, Jianfeng Ping, Jian Wu* and Yibin Ying. Counting DNA molecules with visual segment-based readouts in minutes. Chemical Communications, 2018, 54(9): 1105-1108.

  22. Rui Wang, Xingning Xiao, Yuan Chen, Jun Wu, Wenjuan Qian, Liu Wang, Yuan Liu, Feng Ji and Jian Wu*. A loop-mediated, isothermal amplification-based method for visual detection of Vibrio parahaemolyticus within only 1 h, from shrimp sampling to results. Analytical Methods, 2017, 9(11): 1695-1701.

  23. Rui Wang, Fang Zhang, Zunzhong Ye, Jian Wu*, Liu Wang, Yuan Chen, Xiaofu Wang and Junfeng Xu. Portable and Amplicon Contamination Prevention Cartridge for DNA Amplification Coupled to Lateral Flow Detection. Analytical Methods, 2015, 7(9): 3692-3696.

  24. Rui Wang, Jian Wu*, Fang Zhang, Liu Wang and Feng Ji. On-point Detection of GM Rice in 20 Minutes with Pullulan as CPA Acceleration Additive. Analytical Methods, 2014, 6(23), 9198–9201.

  25. Rui Wang, Yuan Chen, Kai Fan, Feng Ji, Jian Wu, Yonghua Yu*. Nominal effective immunoreaction volume of magnetic beads at single bead level. J. Zhejiang Unvi-SCB, 2017, 18(10), 845-853.

  26. Junhao Xie#, Xianzhu Zhou#, Rui Wang#, Jiulong Zhao, Jian Tang, Qichen Zhang, Yiqi Du and Yanan Pang*. Identification of potential diagnostic biomarkers in MMPs for pancreatic carcinoma. Medicine, 2021, 100(23):e26135.

  27. Qiqi Cai, Rui Wang, Zhaohui Qiao* and Wenge Yang. Single-digit Salmonella detection with the naked eye using bio-barcode immunoassay coupled with recombinase polymerase amplification and a CRISPR-Cas12a system. Analyst, 2021, 146, 5271.

  28. Cui Wu, Zhen Wang, Hui Wu, Zhan Lu, Cheng Qian, Shunru Jin, Rui Wang, Youqing Meng, Jian Wu, Jianfeng Ping, Yibin Ying, Zunzhong Ye*, Transactions of the ASABE, 2021, 64(1): 95-102.  

  29. Qiqi Cai, Hanxing Shi, Mengni Sun, Na Ma, Rui Wang, Wenge Yang, Zhaohui Qiao*. Sensitive Detection of Salmonella Based on CRISPR-Cas12a and the Tetrahedral DNA Nanostructure-Mediated Hyperbranched Hybridization Chain Reaction. Journal of Agricultural and Food Chemistry. 2022, 70(51):16382-16389. (中科院一区TOP，IF=5.7)

  30. Liu Wang, Linlin Bai, Kaiyu He, Rui Wang, Qiang Wang, Fang Zhang, XiahongXu. Graphene oxide assisting the visual detection of salmonella by CRISPR/Cas12a. Microchemical Journal, 2023, 191(7):108870.(中科院二区TOP，IF=5.3).